plotTwist: Visualizing Time-Lapse Data with Condition Indicators

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Last accessed Jul 03, 2026
Author Joachim Goedhart Quantitative biology

About the app

plotTwist is an app for visualizing the data and statistics of continuous data, with a focus on time-dependent measurements. The philosophy of the approach is that plotting the raw data (instead of a summary) improves transparency and interpretation. To facilitate the visualization of individual measurements, the data can be shown as 'small mutliples' or in 'heatmap'. To further facilitate the comparison, the mean and 95% CI can be added. The user has full control over the visibility of the raw data and statistics by adjustment of the transparency (alpha). Several color blind friendly palettes are available to label the data. The comparison of multiple conditions is enabled by multiple file upload , where each file represents a condition.There are several options for annotating the plot, e.g. to indicate the time and nature of a perturbation. This will overlay a transparant grey box, add a bar on top of the graph or both. Original publication: PLoS Biol . 2020 Jan 13;18(1):e3000581.

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